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. Author manuscript; available in PMC: 2020 May 30.
Published in final edited form as: Cell Rep. 2020 Apr 28;31(4):107566. doi: 10.1016/j.celrep.2020.107566

Figure 2. Cross-Reactivity of DENV2-Primed CD4+ T Cells to HLA-DRB1*0101-Binding ZIKV Peptides.

Figure 2.

Ifnar1−/−HLA-DRB1*0101 mice were infected intraperitoneally with 2 × 103 FFU of DENV2 strain S221 for 7 days. Splenocytes were stimulated in vitro for 6 h with the indicated ZIKV-derived peptides that were predicted to bind HLA-DRB1*0101, and the frequencies of cytokine-producing cells were detected using the ICS assay.

(A) Representative flow cytometry contour plots.

(B and C) Quantification of CD4+ T cells producing IFNγ, TNF, or IFNγ plus TNF (B) or IL-2 (C). Data represent the mean ± SEM of three independent experiments (n = 3–4 mice/experiment; total 10 mice). **p < 0.01, ***p < 0.001, ****p < 0.0001 by one-tailed Mann-Whitney test with a B-H adjusted p value (FDR) < 10%. Negative and positive refer to cells incubated in medium alone or with PMA and ionomycin, respectively.