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. Author manuscript; available in PMC: 2020 May 30.
Published in final edited form as: J Cell Physiol. 2018 Sep 7;234(5):6244–6253. doi: 10.1002/jcp.27355

Figure 2. Epigenetic marks associated with the Runx2 P1 promoter in MC3T3 cells differentiating to osteoblasts.

Figure 2.

(A) Runx2/p57 (left) and Bglap (right) mRNA levels are significantly upregulated during osteoblastic differentiation. MC3T3 cells treated with Ascorbic Acid (AA) 50ug/mL were collected at the indicated days and mRNA levels analyzed by RT-qPCR. mRNA expression values were normalized against Gapdh mRNA levels. (B) Alkaline phosphatase staining to determine osteoblast extracellular matrix maturation. (C-G) ChIP assays using antibodies against (C) H3Ac, (D) H3K4me3, (E) H3K27Ac, (F) H3K27me3 and (G) H4R3me2s performed at the indicated days of differentiation. Results are expressed as % Input ± SEM. Normal IgG was used as specificity control. Statistical analyses were performed with respect to 3 DIV. ***p<0.001, **p<0.01, ns = non-statistically significant differences.