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. Author manuscript; available in PMC: 2020 May 30.
Published in final edited form as: J Cell Physiol. 2018 Sep 7;234(5):6244–6253. doi: 10.1002/jcp.27355

Figure 4. Knockdown of Wdr5 and Utx expression inhibits Runx2/p57 transcription in differentiating osteoblasts.

Figure 4.

(A-B and D-E) Differentiating MC3T3 cells (3DIV) were infected with lentiviral particles containing shRNAs against the chromatin modifiers: (A) Wdr5, (B) Utx, (D) Ezh2 and (E) Prmt5. Knockdown efficiencies were confirmed by RT-qPCR and western blot (A-D, left panels) analyses, 48 h post-infection (5 DIV). TFIIB or RNAPII proteins were used to control for equal protein loading. (C) Effect of knocking down Wdr5 (left) and Utx (right) expression on Bglap gene transcription. mRNA expression values were normalized against Gapdh mRNA levels. Statistical analyses were assessed with respect to the mRNA levels obtained in cells infected with an empty vector (EV). *p<0.05, ***p<0.001, ns = non-statistically significant differences.