Skip to main content
. Author manuscript; available in PMC: 2020 May 30.
Published in final edited form as: J Cell Physiol. 2018 Sep 7;234(5):6244–6253. doi: 10.1002/jcp.27355

Figure 5. Runx2/p57 transcription is independent of Jarid1b function at the P1 promoter in differentiating osteoblasts.

Figure 5.

MC3T3 (3DIV) cells were infected with lentiviral particles containing shRNAs against Jarid1b. Knockdown efficiency was determined 48 h later by RT-qPCR and western blot. RNA-Polymerase II protein was determined to control for equal loading. Runx2/p57 mRNA levels were measured by RT-qPCR normalizing against Gapdh. Statistical analyses were performed respect to mRNA levels observed in infected cells with an empty vector (E.V). ***p<0.001.