Figure 3.

Expression of Cas12a crRNA using pol II promoters. (A) New constructs were designed using pol II promoters and terminators to express crRNA. (B) crRNA release from primary RNA transcripts. (C) New endogenous and exogenous pol II promoters tested and their editing efficiency targeting ade6⁺. The same gRNA sequence (gRNA-3) was used here. Error bars represent mean ± S.E.M. using three technical replicates. An unpaired t-test was used to assess the significance of higher efficiency using nmt1 or fba1 promoter compared to the rrk1 promoter (**P < 0.01, ***P < 0.001). The original colony counts were listed in Supplementary Table S8.