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. 2020 May 6;48(10):5788–5798. doi: 10.1093/nar/gkaa329

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Multiplex genome editing using crRNA arrays. (A) The crRNA arrays designed to express multiple crRNA on a single transcript. (B) Genome editing efficiency using rrk1 or fba1 promoter to drive the crRNA array expression, with their mean efficiency ± S.E.M. Gray: positive editing efficiency at ade6 alone using array-1. Orange: double positive editing efficiency at ade6 and leu1, using array-2. Blue: triple positive editing efficiency at ade6, leu1 and his3, using array-3. (C) The transformant count for ade6 or leu1 deletion from the group using array-2 driven by fba1 promoter.