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CRISPR interference using dCas12a. (A) The dCas12a with D917A mutation was used to reduce expression of ade6+. (B) One gRNA proximal to TSS was used to repress the ade6 expression. (C) Spot assay to check CRISPRi repression using dCas12a alone or dCas12a-Clr4. (D) CRISPRi repression of ade6 using dCas12a fused to the Clr4 catalytic domain or catalytic dead mutants.
