Figure 1.

Targeting the HIV genome with single crRNAs. (A) The crRNAs were designed to target the sense or antisense strand of HIV proviral DNA. (B) The activity of anti-HIV crRNAs to silence HIV DNA. Plasmids encoding HIV LAI and Cas12a/crRNA were co-transfected into HEK293T cells. Two days post-transfection, culture supernatant was collected for CA-p24 ELISA to measure viral gene expression. The dash line reflects 50% knockdown based on the CA-p24 value generated from the Ctrl crRNA that does not target HIV. The data represent the mean ± standard deviation (SD) of n = 3 independent biological replicates.