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. 2020 Apr 13;48(10):5527–5539. doi: 10.1093/nar/gkaa226

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Monitoring HIV replication in Cas12a/crRNA expressing SupT1 cells. Cells were infected with 1 ng CA-p24 HIV (at day 0) and cultured for up to 60 days. The day at which massive virus-induced syncytia was observed (representing breakthrough HIV replication) was scored in 6 parallel cultures after infection (day 0). A single TatRev culture was lost due to bacterial contamination. The cultures without virus replication until day 60 were marked as No breakthrough HIV replication (black box). SupT1, non-transduced cells; Ctrl, cells transduced with a control crRNA that does not target HIV. The data reflects mean values ± SD.