Figure 5.

Indel pattern upon CRISPR attack. The frequency of WT HIV target sequences and different types of mutations (regular deletion and insertion, and the new delin class) for Cas9 (left) and Cas12a (right panel). This analysis was performed for HIV-infected T cell cultures that were collected at 110 days (Cas9 attack with two gRNAs) or 60 days (Cas12a attack with an individual crRNA). We analyzed 125 sequences for Cas9 (Supplementary Figure S2) and 114 sequences for Cas12 (Supplementary Figure S1, Figure 3 shows a subset of these sequences).