Table 2.

Relevant modified motifs detected in B. subtilis by PacBio SMRT sequencing

Motifa	Type	%Detected	Mean QV	Mean Cov.	Mean IPD ratio
ΔdnmA WT PY79
CTCGARB	m5Cb	46.7	51	120	2.00
ΔdnmA WT 3610
Nonec				358
ΔdnmA, amyE::Pspac dnmA PY79
GACGAG	m6A	99.7	213	152	6.32
CTCGARB	m5C	52.7	59	149	2.00

^aAll motif calls by SMRT sequencing are reported in Supplementary Table S6.

^bModification type confirmed via methylation sensitive restriction endonuclease treatment as described in the supporting document.

^cGACGAG and CTCGARB were not detected in NCIB 3610 ΔdnmA. All other motifs called are reported in Supplementary Table S6. The average coverage is reported for each spurious motif detected.