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. 2020 Apr 24;48(10):5254–5267. doi: 10.1093/nar/gkaa281

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Testing of self-biotinylation by various GQ-forming DNAs (A) and RNAs (B) as well as by a DNA (‘BLD’) and an RNA (‘r(ssRNA)’) that do not fold to GQ. Both panels A and B show 7.5% non-denaturing gels run in 50 mM TBE buffer at room temperature. ‘StAv’ refers to StAv, where it has been added subsequent to the standard biotinylation reaction (marked ‘+’) or where it has not been added (marked ‘−’). The percent values shown in a given ‘+’ lane refer to the percentage of DNA in that lane that has been mobility-shifted by virtue of complexation of the biotinylated DNA with the added StAv.