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. 2020 Apr 24;48(10):5254–5267. doi: 10.1093/nar/gkaa281

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — How many biotins attach to each ‘CatG4’ GQ? (A) Design of the reference complex between a synthetic, singly biotinylated ‘CatG4’ DNA and its 1:1 complex with monoavidin(MAv). (B) Expected 1:1 complexation of monoavidin with biotin moieties attached to a ‘CatG4’ DNA via peroxidation of a BT substrate by the ‘CatG4’s’ oxidative complex formed with hemin. (C) Two exposures of a native polyacrylamide gel showing the appearance of up to four monoavidin-retarded bands (shown with red asterisks) formed from the reaction described in (B).