Figure 3.

Subunit requirements for H3K4 methylation activities of human MLL1^Core and MLL2^Core complexes. (A) SDS-PAGE/Coomassie blue staining of purified MLL1^Core complexes lacking the indicated subunits. (B) Whole-cell extracts and purified MLL1^Core complexes prepared from Sf9 cells infected with baculoviruses in the absence of the indicated subunits were subjected to immunoblot analyses as in Figure 2B. (C and D) H3 (C) and H2Bub chromatin (D) substrates were subjected to in vitro HMT assays with subunit-lacking MLL1^Core (left) or MLL2^Core (right) complexes (15 nM).