Figure 1.
Cohesin SA1 and SA2 bind to single-stranded RNA in a length dependence manner. (A and B) Binding of SA1 (A) and SA2 (B) to 25, 45 and 66 nt ssRNA measured by fluorescence anisotropy using fluorescein-labeled RNA. The data were fitted to the law of mass action (R2 > 0.99). The error bars (standard deviations) are from three measurements. The RNA oligo sequences are shown in Supplementary Table S1. The equilibrium dissociation constants (Kd) were calculated from at least two independent experiments (Table 1).