Figure 2.
SA1 and SA2 bind to double-stranded substrates containing RNA. (A) Schematic illustration of double-stranded substrates containing RNA that were used for fluorescence anisotropy experiments. The green star represents the 5′ fluorescein label. The RNA oligo sequences are shown in Supplementary Table S1. (B and C) Concentration-dependent binding of SA1 (B) and SA2 (C) to dsRNA, dsRNA with an overhang, and RNA:DNA hybrids with or without overhangs. The data were fitted to the law of mass action (R2 > 0.96). The error bars (standard deviations) are from three measurements. The equilibrium dissociation constants (Kd) were calculated from at least two independent experiments (Table 1).