Table 1.
The equilibrium dissociation constants (Kd) of SA1, SA2 and EWSR1 for different RNA-containing substrates measured from fluorescence anisotropy experiments
| RNA containing substrates | SA1 Kd [nM] (mean ± SEM) | SA2 Kd [nM] (mean ± SEM) | EWSR1 [nM] (mean ± SEM) | |
|---|---|---|---|---|
| ssRNA | 25 nt (R-loop oligo) | 163.7 ± 37.4 | 251.1 ± 3.2 | 134.2 ± 0.04 |
| 45 nt | 6.0 ± 0.6 | 11.4 ± 1.2 | ||
| 66 nt-1 | 3.2 ± 0.3 | 7.2 ± 2.5 | 35.1 ± 1.8 | |
| 66 nt-2 | 3.0 ± 0.1 | 5.1 ± 0.6 | ||
| dsRNA substrates | dsRNA (66 bp) | 61.5 ± 9.3 | 31.2 ± 1.8 | 34.6 ± 6.9 |
| dsRNA + an overhang | 95.8 ± 6.1 | 56.8 ± 0.5 | 44.0 ± 1.7 | |
| Substrates related to the model R-loop | RNA:DNA hybrid + overhangs | 25.8 ± 3.4 | 23.4 ± 1.3 | 94.7 ± 10.7 |
| RNA:DNA hybrid (25 bp) | 58.6 ±1.5 | 43.3 ± 5.6 | ||
| RNA:DNA hybrid (45 bp) | 44.0 ± 5.9 | 31.7 ± 1.5 | 27.7 ± 4.1 | |
| Model R-loop | 38.4 ± 8.7 | 36.0 ± 4.0 | 28.6 ± 0.8 | |
K d was calculated from two to three independent experiments.