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. 2020 Apr 24;48(10):5670–5683. doi: 10.1093/nar/gkaa267

Figure 1.

Figure 1.

CWC27 is associated with CWC22 and eIF4A3. (A) Volcano plot of protein enrichment for FLAG-CWC27 immunoprecipitation versus control purification. Not-significant proteins (FDR > 0.01) are indicated in light grey. (B) Western blots of proteins coimmunoprecipitated with FLAG-CWC27. Protein extracts from WT and FLAG-CWC27 (FLAG) cells were treated with (+) or without (−) RNase. Detected proteins are indicated on the left. (C) Same as (B), with affinity purified antibodies anti-Rab5, anti-CWC27, anti-CWC22 (N-terminus), anti-CWC22 (C-terminus) and anti-eIF4A3 used for immunoprecipitations with protein extracts from HeLa cells treated with RNase. (D) Enrichment plot of proteins from eIF4A3 and CWC22 purifications. These enrichments were computed using the same control purifications without antibodies during the immunoprecipitation step. Proteins significantly (FDR ≤ 0.01) enriched in anti-CWC22 immunoprecipitation (green), in anti-eIF4A3 (dark gray) or in both (orange) are indicated on the plot. Not-significant proteins are indicated in light gray.