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. 2020 May 4;48(10):5511–5526. doi: 10.1093/nar/gkaa211

Figure 5.

Figure 5.

PRMT7 knockout affects target RBP expression in vivo. (A) Expression levels of three endogenously-tagged RBPs, Torus, RBP16 and DDX3, are altered in Δprmt7 (Δ7) parasites. Lanes are labeled for logarithmic (log) versus stationary (stat) phase promastigotes. EF1a levels are shown as a loading control. (B) HA-RBP16 protein stability was analyzed in log and stationary promastigotes after cycloheximide (CHX) addition. Ponceau staining is shown as loading control. HA-RBP16/Ponceau ratio values are shown below each lane. HA-RBP16 protein half-life was >6 h in WT cells and 0.86 h in Δprmt7 according to a one-phase and a two-phase decay exponential fit, respectively.