Table 1.
Peptides used in the different experiments
| Cell penetrating peptides | Sequence | Used in experiments |
|---|---|---|
| RWLVK | Ac-MD-RWLVK-WKKKRKI-RRRRRRRRRRR | MIC-assay, RifR assay, MST |
| RWLVK* | Ac-MD-RWLVK-GILQWRKI-RRRRRRRRRRR | MIC-assay, DNA replication assay, RifR assay |
| RFSLK | Ac-MD-RFSLK-WKKKRKI-RRRRRRRRRRR | MIC-assay, Mutation frequency (GenR) assay, RifR assay, In vivo skin model, MST |
| RFSLK* | Ac-MD-RFSLK-GILQWRKI-RRRRRRRRRRR | MIC-assay |
| RALVK | Ac-MD-RALVK-WKKKRKI-RRRRRRRRRRR | MIC-assay, RifR assay, MST |
| RWLK | Ac-MD-RWLK-WKKKRKI-RRRRRRRRRRR | MIC-assay, RifR assay, MST |
| R11 | RRRRRRRRRRR | MIC-assay, DNA replication assay, RifR assay, MST |
| Overexpressed peptides | ||
| RWLVK | MD-RWLVK- GILQSTLA | RifR assay, Overexpression (viability) assay |
| RFSLK | MD-RFSLK- GILQSTLA | RifR assay, Overexpression (viability) assay |
| RALVK | MD-RALVK- GILQSTLA | Overexpression (viability) assay |
| RWLK | MD-RWLK- GILQSTLA | RifR assay, Overexpression (viability) assay |
| Octamer peptides | ||
| RWLVK | Ac-MDRWLVKW | SPR analysis, NMR spectroscopy |
| RALVK | Ac-MDAWLVKW | SPR analysis, NMR spectroscopy |
| Pol III peptide | Ac-EQVELEFD | NMR spectroscopy |
* = different linker between APIM motif and cell penetrating part (R11). The C-termini on all peptides were amidated. All peptides were TFA-salt (>90% pure). Lyophilized peptide from the manufacturer was dissolved in stock solutions of 1 mM peptide in water.