. 2020 Apr 29;48(10):5540–5554. doi: 10.1093/nar/gkaa278

Table 2.

APIM-peptide variants reduce spontaneous mutation frequency in E. coli. Overexpression of RWLVK (n = 5), RFSLK (n = 5), RWLK (n = 6) and empty vector/control (n = 12) was conducted in E. coli BL21

	Mutation frequency (Rif^R/10⁸ CFU)
Peptide-variants	Overexpressed peptides	Added peptides
no peptide (control)	2.5 ± 0.4	10.5 ± 2.2
RWLVK	0.02 ± 0.01 **	5.9 ± 2.3 **
RFSLK	0.01 ± 0.003 **	4.1 ± 1.6 *
RWLK	3.8 ± 1.2	7.7 ± 3.3
R11	na	7.2 ± 3.9

Addition of cell penetrating peptide variants (20 μM) containing RWLVK (n = 11), RFSLK (n = 4), RWLK (n = 7), R11 only (n = 5) and no peptide/control (n = 11) was conducted in E. coli MG1655. Average ± SEM, n = independent biological experiments. Two tailed, paired t-test, P < 0.05*, P ≤ 0.005**.