Table 1.
Data collection, image processing and refinement statistics
| Data collection | |
| Sample support | Quantifoil R1.2/1.3 200 mesh copper |
| Microscope | Titan Krios |
| Detector | K2 Summit post Quantum GIF |
| Voltage (kV) | 300 |
| Pixel size (Å) | 1.06 |
| Box size (pix) | 140 |
| No. movie frames | 38 |
| Exposure time (s) | 7.6 |
| Electron dose (e−/ Å2) | 50 |
| No. of micrographs recorded/used | 2608/1196 |
| Reconstruction | |
| Software | RELION |
| No. of particles picked/used | 227 552/63 968 |
| Symmetry | C1 |
| Final Resolution (Å) | 3.9 |
| B factor (Å2) | 168 |
| Accuracy of rotations (o) | 2.31 |
| Accuracy of translations (pix) | 0.71 |
| Refinement | |
| Software | Phenix |
| Map-sharpening B factor (Å2) | 168 |
| Model Composition | |
| Non-hydrogen atoms | 11 104 |
| Protein residues | 729 |
| DNA base pairs | 130 |
| Average B factor (Å2) | 38.2 |
| Correlation coefficient (around atoms) | 0.774 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.010 |
| Bond angles (o) | 1.09 |
| Ramachandran plot statistics (%) | |
| Favored | 93.3 |
| Allowed | 6.7 |
| Outlier | 0 |
| Molprobity analysis | |
| All-atom clashscore | 3.93 |
| Overall score | 1.62 |
| Rotamer outliers (%) | 0 |
| Cβ outliers (%) | 0 |
| EMRinger score | 2.47 |