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. 2020 May 1;48(10):5407–5425. doi: 10.1093/nar/gkaa292

Table 3.

Primers used in this study

Primer name Primer sequence 5′-3′
Anchored oligo d(T) primer TTTTTTTTTTTTTTTTTTTTVN
Random hexamer NNNNNN
ISM1 Total Forward AGCAAGCGATATCTCGCCAA
ISM1 Total Reverse GTCCATGACAATCCCAGCCA
ISM1 pA4 Forward CACCAAGCATCACCTCCCAT
ISM1 pA4 Reverse ATCCTCTTCGGCTGAGTTGG
FAT1 Total/pA1 Forward TCACGGTGGTTGCCTTGCGT
FAT1 Total/pA1 Reverse TGGATGTGCGTGGCTCCTGT
FAT1 pA2 Forward CAAAGGGTTTGGATGGAAATGACAC
FAT1 pA2 Reverse TCCCAATCAGCAGCGGTCAAG
MDV1 Total/pA1 Forward TCACACAGAGCTTCCTAACTTCCA
MDV1 Total/pA1 Reverse ACCCCAGGCGGTATGAGAAATGA
MDV1 pA2 Forward TGAGGGTCGTGAAAATGGGGAC
MDV1 pA2 Reverse TCTTCAAATGGGTTGACTTGATTGC
RPB2 Total Forward GCCTGTAGAGGGTAGATCGAG
RPB2 Total Reverse TCAGCCCGCAAATACCACAA
RPB2 pA2 Forward TCATTTGTGCTGATCTTGCCA
RPB2 pA2 Reverse TGCTTGAAAGTTCTCTCTGCT
RRD2 Total Forward GGGAAGAATCCCCAACAAGAGC
RRD2 Total Reverse ACTGCTCATCTGTGAGAGAGGG
RRD2 pA2 Forward TCTCCACCAAGAGGCCACATAC
RRD2 pA2 Reverse AGTAGCCGCAATAGCGCTCG
RAD53 Total/pA1 Forward ACCAAACCTCAAAAGGCCCCGA
RAD53 Total/pA1 Reverse AGGGGCAGCATTTTCTATGGGT
RAD53 Long Isoform/Between pAs Forward AACCCGTCTTATGCCTTCCGGG
RAD53 Long Isoform/Between pAs Reverse GCCGCCTCCGCCCCTTAATC
PDC1 Forward GCCAGTCTTCGATGCTCCAC
PDC1 Total Reverse ATCGCTTATTGCTTAGCGTTGG
PDC1 pA Span Reverse ACTGTCGGCAACTTCTTGTCTGG
RPP1B Total Forward ACGCTAAGGCTTTGGAAGGTAAGGA
RPP1B Total Reverse AACCGAAACCCATGTCGTCGTCAGA
RPP1B pA Span Forward GACGACGACATGGGTTTCGGT
RPP1B pA Span Reverse TCGTAGCCCTTTCGTATGGACA
RAD53 Promoter Forward AGGTAAGAAAGCAGAAAAGGACGG
RAD53 Promoter Reverse GCGTGGATTGCTGTGTGGGT
RAD53 CDS Forward TCCTAACGGGCCACTTACCTTT
RAD53 CDS Reverse GGGCCCTTCATGATATGAGCCTCT
RAD53 End of CDS Forward GTCGGCTAAGAAGCCGCCAG
RAD53 End of CDS Reverse CGGGGCCTTTTGAGGTTTGGTC
RAD53 pA2 Forward AGAAGTTTGGGTAATTCGCTGCT
RAD53 pA2 Reverse TCTTCCCTTACGTGGTAGGC
GRS2 Total Forward ATAACGATGGCTTCCCCGCT
GRS2 Total Reverse ACGTAAAGCCTGCGAGATCC
GRS2 pA2 Forward ACAACCCTGATGAATCGGACTGGG
GRS2 pA2 Reverse ACAGGCGACAGTCCAAATGTTGAT
RTG2 Total Forward AGGGTGGTGTTCGAGAGGGTTC
RTG2 Total Reverse AATGGAGCATAAGGACGGGACGC
RTG2 pA2 Forward AGTGCTTCCGTTCGTTCCAGA
RTG2 pA2 Reverse TGCACGCCAATTTTAACCCTCTCT
18S Forward GATGCCCTTAGACGTTCTGG
18S Reverse GGCCTCACTAAGCCATTCAA