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. 2020 Apr 27;48(10):5397–5406. doi: 10.1093/nar/gkaa290

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Gene 0.3 is required for productive infection of BREX+ culture by bacteriophage T7. Growth curves of BREX- (A), EcoKI+ (B) and BREX+ (C) cell cultures infected with wild-type, 0.3–0.7 fusion, or Δ0.3 T7 phages at MOI = 0.001 are shown. (‘BREX-’ is an Escherichia coli BW25113 with an empty pBTB-2 vector; ‘BREX+’ carries a full E. coli HS BREX gene cluster inserted into the pBTB-2 vector; and ‘EcoKI+’ is E. coli AB1157 carrying a genomic copy of type I EcoKI R–M system). Phage was added at t = 0, and each growth curve represents the mean optical density and standard deviation values obtained from three independent experiments.