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. 2020 Apr 27;48(10):5397–5406. doi: 10.1093/nar/gkaa290

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Levels of protection against T7 and its derivatives afforded by BREX and EcoKI systems. Level of protection is shown as the ratio between the titers of lysates of indicated phages obtained on non-restrictive (BREX–) relative to restrictive (BREX+ or EcoKI+) strains. In the case of the T7 Δ0.3 phage, no plaques were detected on the BREX+ lawn, and the level of protection was calculated on the basis of appearance of zones of inhibited cell growth at certain phage concentrations. Mean values and standard deviations obtained from three independent experiments are shown. Plates are presented in Supplementary Figure S3.