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. 2020 May 8;48(10):5201–5216. doi: 10.1093/nar/gkaa304

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Different length ribosome footprints represent different translational states. Meta-gene plots, from flash frozen and no drug exposure Hv cultures, of ribosome density mapped using the (A) 5′-end and (B) 3′-end of ribosome footprints (rpm = reads per million). Heatmaps of footprint density separated by length are aligned underneath the meta-gene plots. (C) 3′-mapped meta-gene analysis of small footprints (10–20 nt) and (D) of 27 nt footprints from flash frozen and no drug cultures. (E) Footprint length distribution of 5′-mapped ribosome density for initiating ribosomes at the start codon (at P-site) in leadered (5′ UTR ≥ 10 nt) and leaderless (0 nt) transcripts (left, red) and elongating ribosomes within the open reading frames (right, yellow). (F) 27 nt footprints on leadered (5′ UTR ≥ 10 nt) and leaderless (0 nt) messenger RNAs from flash frozen and anisomycin (ANS) treatment prior to harvesting. (G) A model for footprint lengths corresponding to ribosome translation states. Small footprints (∼15–16 nt) correspond to initiation on leaderless transcripts, and 27 nt footprints correspond to initiation on leadered transcripts and to elongation on all transcripts.