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. 2020 May 8;48(10):5201–5216. doi: 10.1093/nar/gkaa304

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — H. volcanii global ribosome pausing. (A) Pause scores for all footprints were calculated for each codon and averaged per amino acid at the A- (colored large dot), P- (small gray dot), and E- (small black dot) sites of ribosomes for serine hydroxamate (SHX) treated cells. Ribosome sites were calculated from the 3′ of elongating footprints. (B) Meta-gene plots of ribosome density for elongating footprints (27 nt) for methionine and serine codons. (C) Northern blots of charged and uncharged Met or Ser tRNAs from total RNA of SHX-treated cells. Deacylation treatment is indicated by + and charged/uncharged tRNAs are marked. (D) Comparative heatmap of pause scores for all amino acids under several conditions for A-site, P-site, and E-site of the ribosome.