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. 2020 Apr 27;48(10):5710–5719. doi: 10.1093/nar/gkaa295

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — HnRNP C promotes CELF2 mRNA expression while CELF2 promotes hnRNP C protein expression. (A) Western blot of CELF2 and hnRNP C upon shRNA-mediated knock-down of either protein in PMA-stimulated Jurkat cells. HnRNP L is used as a loading control. (B) Quantification of protein expression 48 h after knock-down, as shown in representative blots in panel A and Supplemental Figure S2. Values are from three biologically independent experiments and normalized to the expression of each protein in unstimulated wildtype cells. (C) Quantification of CELF2 and hnRNP C mRNA by qPCR from unstimulated or PMA-stimulated wildtype Jurkat cells compared to those depleted of CELF2 or hnRNP C for 48 hours. ND indicates mRNA was below the level of detection. Double asterisk indicates P< 0.01.