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. 2020 May 1;48(10):5426–5441. doi: 10.1093/nar/gkaa316

Figure 2.

Figure 2.

Analysis of Yap8 basic region variants. (A) Mutagenesis strategy to stepwise turn the Yap8 basic region into Yap1-like sequences. (B) β-galactosidase activity driven by the ACR3-lacZ promoter was measured in the yap1Δ yap8Δ mutant expressing either Yap1, Yap8 or Yap8 mutant proteins. Cells were exposed to 0.1 mM As(III) for 6 h or left untreated for the control. The values are the means of three biological replicas performed in triplicate ± S.D. (C) Complementation of As(III) sensitivity of yap8Δ by Yap8 variants. The yap8Δ mutant was transformed with empty vector (pYX122) or plasmids expressing indicated Yap8 variants. The resulting transformants were spotted on minimal selective plates containing various concentrations of As(III) and incubated 3 days at 28°C. (D) β-Galactosidase activity driven by the MUT3_ACR3-lacZ promoter was measured in the yap1Δ yap8Δ mutant expressing either Yap1, Yap8 or Yap8 mutant proteins. Cells were exposed to 0.1 mM As(III) for 6 h or left untreated for the control. The values are the means of three biological replicas performed in triplicate ± S.D.