Table 1.

Binding affinity (K_d) of the Yap8 protein and its mutant variants to DNA fragments corresponding to the Y8RE-containing ACR3 promoter region

Protein	K d (nM)	DNA probe
Yap8 (WT)	9.9 ± 1.4a	ACR3-WT
Yap8-7aa	9.3 ± 1.7a	ACR3-WT
Yap8-8aa	15.1 ± 2.1a	ACR3-WT
Yap8-N20A	25.5 ± 4.3a	ACR3-WT
Yap8(WT)	10.8 ± 1.7b	ACR3-WT
Yap8-R7A	13.4 ± 1.7b	ACR3-WT
Yap8-R11A	18.9 ± 2.9b	ACR3-WT
Yap8 (WT)	10.8 ± 3.5c	ACR3-WT
Yap8 (WT)	19.4 ± 4.1c	ACR3-M3

The K_d values of dissociation constant were derived after curve fitting to anisotropy binding data shown in Figure 3C^a, Figure 5E^b and Figure 8C^c, respectively.