Table 2.
Summary of functional analysis of Yap8 mutant proteins
| Mutant | Mutated | As(III) | Mutant | Model |
|---|---|---|---|---|
| Name | Region | Resistance | Class | Prediction |
| P4A | N-term | +++ | F | Not included in the model |
| R5A | N-term | + | PF | Not included in the model |
| G6A | N-term | + | PF | Not included in the model |
| R7A | N-term | + | PF | interacts with DNA bases and backbone |
| K8A | N-term | +++ | F | Residue's backbone interacts with DNA backbone |
| G9A | N-term | + | PF | Plasticity of N-term region, tighter contact with DNA |
| G10A | N-term | − | NF | Plasticity of N-term region, tighter contact with DNA, interacts with DNA backbone |
| R11A | N-term | + | PF | Interacts with DNA backbone |
| K12A | N-term | +++ | F | Interacts with DNA backbone |
| P13A | N-term | +++ | F | Model shows no contact with DNA |
| S14A | N-term | +++ | F | Interacts with DNA backbone |
| L15A | N-term | +++ | F | Model shows no contact with DNA |
| T16A | N-term | +++ | F | Model shows no contact with DNA |
| P17A | N-term | +++ | F | Model shows no contact with DNA, caps the basic region |
| P18A | N-term | +++ | F | Model shows no contact with DNA, caps the basic region |
| K19A | N-term | +++ | F | Some contact with DNA backbone |
| N20A | N-term | + | PF | No contact with DNA, defines the conformational space of N-terminal region to enable tighter protein-DNA contacts |
| K21A | N-term | +++ | F | Interacts with DNA backbone, seen only for monomer 2 |
| R22A* | Basic | − | NF | Direct contact with TGA bases of Y8RE |
| A23 | Basic | ND | ND | Model shows no contact with DNA |
| A24 | Basic | ND | ND | Model shows no contact with DNA |
| Q25A | Basic | − | NF | Interacts with DNA backbone |
| L26A* | Basic | − | NF | Model shows no contact with DNA, but this observation is sensitive to definition of a hydrophobic interaction |
| R27A* | Basic | ++ | PF | Interacts with DNA backbone |
| A28 | Basic | ND | ND | Model shows no contact with DNA |
| S29A* | Basic | +++ | F | Interacts with DNA backbone |
| Q30 | Basic | ND | ND | Forms several H-bonds with bases from the central region of Y8RE |
| N31A* | Basic | +++ | F | Some contact with DNA backbone |
| A32 | Basic | ND | ND | Model shows no contact with DNA |
| F33 | Basic | − | NF | Hydrophobically interacts with TGATT |
| R34 | Basic | ND | ND | Interacts with DNA backbone and central A base of Y8RE |
| K35A | Basic | +++ | F | Model shows no contact with DNA |
| R36A | Basic | ++ | PF | Direct contact with DNA backbone |
| K37A | Basic | +++ | F | Model shows no contact with DNA |
| L38A | Basic | +++ | F | Model shows no contact with DNA |
| E39A | Basic | +++ | F | Model shows no contact with DNA |
| R40A | Basic | ++ | PF | Model shows no contact with DNA |
Mutated region: N-term refers to N-terminal region preceding the basic region. As(III) resistance: −, none; + or ++, partial; +++, full. Mutant class: F – functional, PF – partially functional, NF – non-functional. ND – not determined. *Determined by Amaral et al. (18).