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CD thermal denaturation profiles of the hTERT WT and antiparallel hairpin sequences. (A) Strand-normalized CD spectra from 220 to 320 nm over 4–98°C for the WT and AH sequences annealed in potassium phosphate buffer with 200 mM KCl (left) with normalized melting curves (right). (B) Strand-normalized CD spectra (left) and melting curves (right) for WT and AH sequences annealed in phosphate buffer with 10 mM KCl.
