Table 2.
Partial list of differentially expressed genes for Δ6/pCA24N-dinJ versus Δ6/pCA24N.
| Group and gene name | b number | Fold change | Description of encoded protein |
|---|---|---|---|
| Stress response | |||
| osmB | b1283 | −3.0 | Osmotically and stress inducible lipoprotein |
| cspE | b0623 | −3.5 | DNA-binding transcriptional repressor, cold-shock protein |
| umuC | b1184 | 3.7 | SOS mutagenesis and repair, DNA polymerase V, subunit D |
| umuD | b1183 | 4.0 | DNA polymerase V subunit, error-prone repair |
| ybeS | b0646 | 3.0 | Protein folding, heat shock protein binding |
| ygeG | b2851 | 3.0 | Response to stress |
| Cell wall, membrane and motility/chemotaxis | |||
| csgB | b1041 | 2.5 | Curlin nucleator protein, homology with major curlin, CsgA |
| eaeH | b0297 | 2.5 | Cell adhesion |
| fliC | b1923 | −6.1 | Flagellar filament structural protein (flagellin) |
| fliK | b1943 | 3.2 | Flagellar hook-length control protein |
| yaiC | b0385 | 2.8 | Cellulose, biofilm, motility regulator, diguanylate cyclase; CsgD-regulated |
| yehD | b2111 | 2.6 | Predicted fimbrial-like adhesin protein |
| yfjP | b2632 | 3.0 | Required for swarming phenotype |
| DNA replication, recombination and repair | |||
| oraA | b2698 | 3.0 | Blocks RecA filament extension; inhibitor of RecAATPase, reduced resistance to DNA damage |
| recN | b2616 | 4.6 | DNA Recombination and repair |
| rusA | b0550 | 2.6 | DNA repair Endonuclease |
| yeeS | b2002 | 2.6 | CP4-44 prophage; predicted DNA repair protein |
| Transport | |||
| fruB | b2169 | 9.8 | Fructosephosphotransferase enzyme III |
| yddL | b1472 | 4.0 | Transport, OmpCFN porin family, N-terminal fragment |
| Metabolism | |||
| fruK | b2168 | 4.0 | Fructose-1-phosphate kinase; 1-phosphofructokinase |
| ybdK | b0581 | 4.3 | Glutathione biosynthesis, cysteine ligase activity |
Cells at 37°C had DinJ produced for 2 h via 1 mM IPTG then were adjusted to a turbidity of 1.0 and were exposed to erythromycin stress for 10 min in LB low-salt medium. The cut-off ratio was 2.5-fold.