Representative immunohistochemistry images of (a) GFAP, C3, and S100A10 staining from mPFC brain sections of control and early life 50 Mn treatment groups at 63x magnification; white boxes are enlarged (b) for clarity (scale bars, 10 μm). (c, d) Bar charts show quantified fluorescence intensity of GFAP-colocalized puncta for C3 and S100A10, respectively; data reflect 4 images/animal, where n = 5 animals/treatment group. Data are least squares means ± SEM shown as percent of control values generated from the statistical model that included three treatment groups. Bars with different superscripts are statistically different (p < 0.05) based on Tukey’s multiple comparisons test.