Table 2.
Molecular properties of hirudin variant HV1 and HLF variants HLF1, HLF2, HLF3, and HLF4
| Factor | Length C1-C6 | Acidic/basic | pI value |
| HV1 | 34 | 4/2 | 4.41 |
| HLF1 | 34 | 3/0 | 3.57 |
| HLF2 | 30 | 1/5 | 8.68 |
| HLF3 | 30 | 1/5 | 8.68 |
| HLF4 | 30 | 2/2 | 6.10 |
| Factor | C-terminal tail | Acidic/basic | pI value |
| HV1 | 26 | 8/2 | 3.94 |
| HLF1 | 24 | 13/1 | 3.26 |
| HLF2 | 18 | 6/2 | 3.85 |
| HLF3l | 21 | 3/6 | 9.40 |
| HLF3s | 4 | 0/2 | 8.76 |
| HLF4a | 9 | 0/4 | 11.10 |
| HLF4b | 15 | 6/4 | 4.53 |
C1-C6 indicates the number of amino acid residues between the cysteine residues 1 and 6 (including C1 and C6). C-terminal tail indicates the number of amino acid residues starting immediately after C6. The ratio acidic/basic indicates the number of acidic and basic amino acid residues within the respective region together with the deduced pI value