Fig. 1.

Cytotoxicity of H₂O₂ and verification of recombinant plasmid expression in HL7702 cells. Cells were treated with different concentrations of H₂O₂, as indicated for 12 h. Cell viability was evaluated using a Cell Counting Kit-8 assay, n = 4 (a). HBV X antigen (HBx) and C antigen (HBc) were transiently expressed in HL7702 cells and protein expression levels determined by western blotting, n = 4 (b, c). Abbreviations: pNC, cells transfected with control vector pcDNA3.1; pHBx, cells transfected with reconstituted pHBx vector; pGEM, cells transfected with control vector pGEM-4Z; pHBV-HBx, cells transfected with pHBV-HBx vector; pHBV, cells transfected with pHBV vector. Data are displayed as mean ± SD. *P < 0.05 and **P < 0.01