Table 1.
Limit of detection for RNA transcripts, spiked jejunum and fecal samples, intra and inter-assay mean Ct, standard deviation (SD), and coefficient of variation (CV) for each point is calculated. In cases where the confidence level did not reach 95%, the number of positives from the number tested are shown.
| Matrix | Viral RNA | Assay 1 | Assay 2 | Assay 3 | Interassay |
|---|---|---|---|---|---|
| Copies / 5 μl | mean Ct ± SD(CV) | mean Ct ± SD(CV) | mean Ct ± SD(CV) | mean Ct ± SD(CV) | |
| RNA transcript | 400 | 32.45 ± 0.13 (0.004) | 31.14 ± 0.13 (0.004) | 30.69 ± 0.11 (0.004) | 31.44 ± 0.7 (0.022) |
| 200 | 33.93 ± 0.69 (0.02) | 31.84 ± 0.22 (0.007) | 31.75 ± 0.2 (0.006) | 32.51 ± 1.38 (0.042) | |
| 100 | 35.61 ± 0.46 (0.013) | 33.42 ± 1.08 (0.032) | 32.65 ± 0.27 (0.008) | 33.89 ± 2.18 (0.064) | |
| 50 | 36.24 ± 0.58 (0.016) | 34.3 ± 0.59 (0.017) | 34.11 ± 0.45 (0.013) | 34.82 ± 1.43 (0.041) | |
| 25 | 7+/8 | 6+/8 | 8+/8 | / | |
| 12.5 | 3+/8 | 3+/8 | 3+/8 | / | |
| Jejunum | 108 | 14.23 ± 0.04 (0.003) | 13.69 ± 0.04 (0.003) | / | 13.96 ± 0.11 (0.008) |
| 106 | 21.52 ± 0.06 (0.003) | 20.77 ± 0.16 (0.008) | / | 21.15 ± 0.25 (0.012) | |
| 104 | 28.55 ± 0.04 (0.002) | 27.57 ± 0.07 (0.002) | / | 28.06 ± 0.29 (0.010) | |
| 102 | 35.17 ± 0.82 (0.023) | 34.75 ± 1.21 (0.035) | / | 34.96 ± 1.05 (0.030) | |
| 50 | 3+/4 | 4+/4 | / | / | |
| Feces | 108 | 12.41 ± 0.22 (0.018) | 13.25 ± 0.20 (0.015) | / | 12.83 ± 0.39 (0.031) |
| 106 | 18.56 ± 0.03 (0.001) | 19.23 ± 0.01 (0.0004) | / | 18.90 ± 0.13 (0.007) | |
| 104 | 25.80 ± 0.14 (0.006) | 26.22 ± 0.14 (0.005) | / | 26.01 ± 0.19 (0.007) | |
| 102 | 32.88 ± 0.45 (0.014) | 32.67 ± 0.52 (0.016) | / | 32.78 ± 0.50 (0.015) | |
| 50 | 34.276 ± 1.07 (0.31) | 33.22 ± 1.11 (0.33) | / | 33.75 ± 1.15 (0.034) |
LoDs determined for each matrix are shown in bold text.