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. Author manuscript; available in PMC: 2021 May 6.
Published in final edited form as: J Am Soc Mass Spectrom. 2020 Apr 2;31(5):1155–1162. doi: 10.1021/jasms.0c00030

Figure 2.

Figure 2.

A selection of proteins and complexes displaying charge states over a wide m/z range, acquired under standard instrument voltages and pressures. All data displayed in magnitude mode using the Hann windowing function of F = 0.5. a) CsI, 50 μg/uL; b) the tetrameric AqpZ complex liberated from a C8E4-micelle, 10 μM (1RC2, downloaded from www.rcsb.org); c) a siRNA-IgG1 conjugate, 5 μM (displaying a single cysteamine modification); d) an IgG1-DM1 conjugate, 5 μM; e) the empty MSP1D1 nanodisc, 10 μM and e) the tetradecameric chaperone complex GroEL, 5 μM (4V43, downloaded from www.rcsb.org). All data acquired over the m/z range 153–20,000 (m/z 1000 to 25,000 for CsI). Transient times ranged from 0.04 s (128 kW) to 0.17 s (512 kW). All spectral zoom-ins and FIDs are displayed in Figures S10 and S11 (Supporting Information).