. Author manuscript; available in PMC: 2020 May 31.

Published in final edited form as: J Med Chem. 2020 Mar 17;63(6):3298–3316. doi: 10.1021/acs.jmedchem.9b02078

Table 1.

Kinetic Parameters of Peptide Substances for Cruzain^a

substrate	structure	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m (μM⁻¹ s⁻¹)	k_cat/K_{m rel}	k_{cat rel}
S1	Cbz-Phe-Arg-AMC	9.6 ± 0.2	0.89 ± 0.09	11 ± 1	1.00	1.00
S2	Cbz-Phe-hPhe-AMC	2.0 ± 0.2	0.26 ± 0.06	8 ± 2	0.73	0.21
S3	Cbz-Leu-hPhe-AMC	3.0 ± 0.2	0.8 ± 0.2	4 ± 1	0.36	0.21
S4	Cbz-Leu-Arg-AMC	8.4 ± 0.4	2.2 ± 0.3	3.8 ± 0.7	0.35	0.88
S5	Cbz-Phe-(4-Pyr)Ala-AMC	7.5 ± 0.5	3.7 ± 0.5	2.0 ± 0.4	0.18	0.76
S6	Cbz-Arg-Arg-AMC^b	7.2 ± 0.1	3.7 ± 0.4	1.9 ± 0.2	0.17	0.75
S7	NMePip-Phe-hPhe-AMC	4.0 ± 0.1	3.1 ± 0.3	1.3 ± 0.1	0.12	0.42
S8	Cbz-(4-Pyr)Ala-hPhe-AMC	5.3 ± 0.2	4.6 ± 0.5	1.1 ± 0.1	0.10	0.55
S9	Cbz-Phe-Phe-AMC	0.26 ± 0.01	0.34 ± 0.06	0.8 ± 0.2	0.07	0.03
S10	Cbz-Arg-hPhe-AMC	4.9 ± 0.1	6.8 ± 0.4	0.7 ± 0.04	0.06	0.51
S11	NMePip-Phe-Phe-AMC	2.8 ± 0.06	14 ± 4	0.2 ± 0.04	0.02	0.29
S12	Cbz-Phe-Ala-AMC^b	0.89 ± 0.06	38 ± 2	0.023 ± 0.003	0.00	0.09

Data obtained at pH 7.5, 25 °C, and 10% DMSO (v/v).

Data obtained at 2% DMSO (v/v) from ref 40.