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. 2019 Mar 18;11(12):1056–1068. doi: 10.1093/jmcb/mjz002

Figure 5.

Figure 5

Morphine inhibits mitophagy by suppressing recruitment of Parkin to the damaged mitochondria and decreasing the ubiquitination of mitochondrial proteins. SH-SY5Y cells were treated with CCCP (10 μM) for 2.5 h with or without morphine (200 μM) for 24 h. (A and C) The levels of Tom20, COX IV, and the phosphorylation of Parkin (Ser65) from whole-cell extract were analyzed by western blotting. (B and D) The levels of Parkin and ubiquitylated proteins in mitochondria were analyzed by western blotting. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001 vs. control group, #P < 0.05, ##P < 0.01 vs. CCCP group. (E) SH-SY5Y cells were treated with CCCP (10 μM) for 2.5 h with or without morphine (200 μM) for 24 h, and then labeled with Mitotracker green and stained with anti-Parkin antibody (red). Scale bar, 10 μm.