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. 2019 Mar 18;11(12):1056–1068. doi: 10.1093/jmcb/mjz002

Figure 7.

Figure 7

Morphine induces the degradation of PINK1 and the rescue of PINK1 improves the accumulation of SQSTM1/p62 induced by morphine. (A) SH-SY5Y cells were treated with CCCP (10 μM) for 2.5 h with or without morphine (200 μM) for 24 h. The level of PINK1 was analyzed by western blotting. n = 3, ***P < 0.001 vs. control group, #P < 0.05 vs. CCCP group. (B and C) SH-SY5Y cells were transfected with pLenti-Ubc-PINK1-2A-EGFP for 48 h, followed by morphine (200 μM) treatment for 24 h. The levels of PINK1 and SQSTM1/p62 were analyzed by western blotting. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001 vs. control group, ##P < 0.01, ###P < 0.001 vs. morphine group.