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. Author manuscript; available in PMC: 2021 Feb 26.
Published in final edited form as: Sci Transl Med. 2020 Feb 26;12(532):eaay7522. doi: 10.1126/scitranslmed.aay7522

Fig. 1. Glioma-associated ECs are chemoresistant.

Fig. 1.

(A to C) GBM was induced by RCAS-mediated somatic gene transfer in Ntv-a;Ink4a-Arf−/−;Ptenfl/fl;LSL-Luc donor mice. Single-cell tumor suspensions were injected into Rosa-LSL-tdTomato;Tie2-Cre or Rosa-LSL-tdTomato;Cdh5-CreERT2 mice. After tumor induction, the mice were treated with saline or with TMZ (100 mg/kg) for 5 days. (A) Schematic approach. (B) Tumor sections were stained with anti-tdTomato and anti-CD31 antibodies. Left: Representative images. Right: Quantified results (n = 4, means ± SEM). Statistical analysis by Student’s t test. (C) Single-cell suspensions isolated from tumors were stained with anti–FSP-1 antibody and analyzed by flow cytometry. Left: Representative sorting. Right: Quantitative data for tdTomato+FSP-1+ and tdTomato+FSP-1− in total cells (n = 5 mice, means ± SEM). Statistical analysis by two-way ANOVA. (D) ECs were treated with TMZ and subjected to cell viability analysis (n = 3, means ± SEM). Statistical analysis by Student’s t test.