Table 2.
Differentially methylated placental CpG sites associated with maternal pre-pregnancy BMI and trimester-specific gestational weight gain
| Traita | CpGa | Chra | Positionb | Relation to Island | Nearest Gene | Feature (or distance from gene)c | % Change in methylation (95% CI) | P | FDR P | BACON corrected FDR P |
|---|---|---|---|---|---|---|---|---|---|---|
| Pre-pregnancy BMI | cg14568196 | chr9 | 139552716 | N_Shore | EGFL7 | 0.6 kb | 0.09 (0.06, 0.12) | 1.41E-10 | 5.79E-05 | 0.01 |
| Pre-pregnancy BMI | cg15339142 | chr12 | 95651472 | OpenSea | VEZT | Body | 0.13 (0.07,0.19) | 3.47E-10 | 7.11E-05 | 0.01 |
| Pre-pregnancy BMI | cg02301019 | chr16 | 85465191 | Island | AC092377.1 | 78.6 kb | −0.07 (−0.09, −0.04) | 1.70E-09 | 0.0002 | 0.02 |
| GWG1 | cg17918270 | chr2 | 1983484 | OpenSea | MYT1L | Body | 24.32 (14.15, 34.48) | 3.30E-09 | 0.0006 | 0.01 |
| GWG1 | cg20735365 | chr7 | 96657023 | S_Shelf | DLX5 | 2.9 kb | −1.01 (−1.47, −0.55) | 1.41E-09 | 0.0005 | 0.01 |
| GWG2 | cg17451688 | chr1 | 234293071 | OpenSea | SLC35F3 | Body | −13.01 (−17.52, −8.49) | 1.20E-08 | 0.002 | 0.04 |
| GWG2 | cg17918270 | chr2 | 1983484 | OpenSea | MYT1L | Body | 28.91 (18.08, 39.73) | 6.36E-10 | 0.0002 | 0.01 |
a
Abbreviations: CpG, Cytosine phosphate Guanine; Chr, chromosome; Pre-pregnancy BMI, Pre-pregnancy body mass index; GWG1, Rate of gestational weight gain at 1st trimester; GWG2, Rate of gestational weight gain at 2nd trimester; kb, distance in kilo bases
b
position of genome build is GRCh37 (hg19)
c
Based on manifest feature annotation from Illumina
For each trait, model was adjusted for offspring sex, maternal age, maternal race/ethnicity, plates, first three principal components from methylation and ten principal components from genotypes.