Table 1:
Effects of Smad2, Smad3 and combined Smad2/Smad3 loss on extracellular matrix gene synthesis by cardiac fibroblasts
| Gene name | Gene symbol | Smad2 KD | Smad3 KD | Smad2+Smad3 KD | |||
|---|---|---|---|---|---|---|---|
| Fold | p-value | Fold | p-value | Fold | p-value | ||
| A disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1motif, 1 | Adamts1 | 1.20 | 0.1026 | 0.57 | 0.0004† | 1.07 | 0.9612 |
| A disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1motif, 2 | Adamts2 | 1.00 | >0.9999 | 0.79 | 0.4414 | 0.95 | 0.9259 |
| A disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1motif, 5 | Adamts5 | 1.79 | 0.0011† | 0.46 | 0.0172† | 1.21 | 0.5691 |
| A disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1motif, 8 | Adamts8 | 0.71 | 0.3369 | 1.99 | 0.0009† | 0.91 | 0.9268 |
| CD44 antigen | Cd44 | 0.85 | 0.0811 | 0.57 | 0.0006† | 0.64 | 0.002† |
| Cadherin 1 | Cdh1 | ND | - | ND | - | ND | - |
| Cadherin 2 | Cdh2 | 0.78 | 0.0617 | 0.77 | 0.0421† | 0.83 | 0.1401 |
| Cadherin 3 | Cdh3 | 0.44 | 0.0342† | 1.40 | 0.2144 | 0.62 | 0.0955 |
| Cadherin 4 | Cdh4 | ND | - | ND | - | ND | - |
| Contactin 1 | Cntn1 | 0.82 | 0.9399 | 0.63 | 0.8804 | 1.31 | 0.9942 |
| collagen type I, alpha1 | Col1a1 | 0.86 | 0.2259 | 0.75 | 0.034† | 0.58 | 0.001† |
| collagen type II, alpha1 | Col2a1 | 0.53 | 0.1216 | 0.42 | 0.0645 | 0.34 | 0.028† |
| collagen type III, alpha1 | Col3a1 | 1.02 | >0.9999 | 0.76 | 0.4218 | 0.99 | 0.99 |
| collagen type IV, alpha1 | Col4a1 | 0.89 | 0.2537 | 0.57 | 0.0001† | 0.79 | 0.0168† |
| collagen type IV, alpha2 | Col4a2 | 0.97 | 0.8428 | 0.86 | 0.2308 | 0.85 | 0.0984 |
| collagen type IV, alpha3 | Col4a3 | 1.23 | 0.9918 | 0.66 | 0.5862 | 0.81 | 0.7044 |
| collagen type V, alpha1 | Col5a1 | 0.59 | 0.0003† | 0.83 | 0.1002 | 0.53 | <0.0001† |
| collagen type VI, alpha1 | Col6a1 | 0.93 | 0.9558 | 0.73 | 0.3592 | 0.98 | 0.9818 |
| Connective tissue growth factor | Ctgf | 0.96 | 0.9705 | 0.73 | 0.1052 | 0.58 | 0.0115† |
| Catenin (Cadherin associated protein), alpha1 | Ctnna1 | 0.96 | 0.8678 | 0.90 | 0.3221 | 1.00 | 0.9981 |
| Catenin (Cadherin associated protein), alpha2 | Ctnna2 | 1.19 | 0.9997 | 0.58 | 0.3288 | 1.03 | 0.8801 |
| Catenin (Cadherin associated protein), beta1 | Ctnnb1 | 1.00 | >0.9999 | 0.95 | 0.8839 | 1.20 | 0.0875 |
| Extracellular matrix protein 1 | Ecm1 | 0.92 | 0.6037 | 1.20 | 0.0914 | 1.26 | 0.0351† |
| Elastin microfibril interfacer 1 | Emilin1 | 1.13 | 0.8245 | 0.75 | 0.2225 | 0.86 | 0.4733 |
| Ectonucleoside triphosphate diphosphohydrolase 1 | Entpd1 | 0.88 | 0.7972 | 0.66 | 0.1511 | 0.90 | 0.9441 |
| Fibulin 1 | Fbln1 | 0.63 | 0.2275 | 0.93 | 0.9068 | 0.90 | 0.8722 |
| Fibronectin 1 | Fn1 | 0.71 | 0.0011† | 0.76 | 0.0061† | 0.63 | 0.0002† |
| Hyaluronan and proteoglycan link protein 1 | Hapln1 | 4.11 | 0.0337† | 3.29 | 0.4624 | 1.20 | >0.9999 |
| Hemolytic complement | Hc | 1.32 | 0.7713 | 1.30 | 0.79 | 0.81 | 0.8101 |
| Intercellular adhesion molecule 1 | Icam1 | 0.66 | 0.0003† | 0.81 | 0.0217† | 0.71 | 0.0011† |
| Integrin alpha2 | Itga2 | 0.72 | 0.1937 | 0.63 | 0.0375† | 0.77 | 0.2425 |
| Integrin alpha3 | Itga3 | 1.23 | 0.5389 | 1.27 | 0.419 | 1.07 | 0.9919 |
| Integrin alpha4 | Itga4 | 0.85 | 0.0618 | 0.54 | <0.0001† | 0.59 | <0.0001† |
| Integrin alpha5 | Itga5 | 0.84 | 0.1638 | 1.07 | 0.689 | 0.90 | 0.5138 |
| Integrin alpha1, epithelial-associated | Itgae | ND | - | ND | - | ND | - |
| Integrin alpha L | Itgal | 2.13 | 0.0003† | 0.39 | 0.0185† | 0.95 | 0.977 |
| Integrin alpha M | Itgam | 1.27 | 0.4943 | 1.33 | 0.3749 | 1.18 | 0.7086 |
| Integrin alpha V | Itgav | 0.86 | 0.1827 | 1.03 | 0.964 | 0.95 | 0.8274 |
| Integrin alpha X | Igtax | 1.42 | 0.278 | 1.30 | 0.5194 | 1.30 | 0.4876 |
| Integrin beta1 | Itgb1 | 0.69 | <0.0001† | 0.96 | 0.7791 | 0.78 | 0.0017† |
| Integrin beta2 | Itgb2 | 1.45 | 0.1434 | 1.06 | 0.983 | 1.12 | 0.8774 |
| Integrin beta3 | Itgb3 | 0.75 | 0.2061 | 0.92 | 0.7783 | 0.85 | 0.3933 |
| Integrin beta4 | Itgb4 | 0.88 | 0.3398 | 0.93 | 0.553 | 0.92 | 0.4494 |
| Laminin,alpha1 | Lama1 | 0.69 | 0.9534 | 0.94 | >0.9999 | 0.59 | 0.6767 |
| Laminin,alpha2 | Lama2 | 1.91 | 0.003† | 1.11 | 0.9619 | 1.36 | 0.2962 |
| Laminin,alpha3 | Lama3 | 0.86 | 0.7634 | 0.37 | 0.2565 | 0.78 | 0.6245 |
| Laminin, beta2 | Lamb2 | 1.36 | 0.2233 | 1.13 | 0.8831 | 1.46 | 0.1447 |
| Laminin, beta3 | Lamb3 | 0.94 | >0.9999 | 0.55 | 0.4203 | 0.65 | 0.5299 |
| Laminin, gama1 | Lamc1 | 1.14 | 0.1251 | 1.06 | 0.6642 | 0.96 | 0.8442 |
| Matrix metallopeptidase 10 | Mmp10 | 0.77 | 0.3023 | 1.21 | 0.9662 | 0.57 | 0.0399† |
| Matrix metallopeptidase 11 | Mmp11 | 1.34 | 0.1113 | 1.42 | 0.0496† | 1.46 | 0.0414† |
| Matrix metallopeptidase 12 | Mmp12 | 1.37 | 0.8689 | 1.90 | 0.2164 | 1.60 | 0.4346 |
| Matrix metallopeptidase 13 | Mmp13 | 1.56 | 0.0317† | 0.54 | 0.0739 | 0.99 | >0.9999 |
| Matrix metallopeptidase 14 | Mmp14 | 1.27 | 0.0205† | 1.31 | 0.0108† | 1.44 | 0.001† |
| Matrix metallopeptidase 15 | Mmp15 | 0.51 | 0.0092† | 1.03 | 0.9927 | 0.72 | 0.1573 |
| Matrix metallopeptidase I, alpha | Mmp1a | 2.23 | <0.0001† | 0.88 | 0.7969 | 1.94 | 0.0004† |
| Matrix metallopeptidase 2 | Mmp2 | 1.42 | 0.0098† | 0.65 | 0.0085† | 0.96 | 0.7422 |
| Matrix metallopeptidase 3 | Mmp3 | 1.52 | 0.5465 | 1.08 | 0.9911 | 2.01 | 0.1364 |
| Matrix metallopeptidase 7 | Mmp7 | ND | - | ND | - | ND | - |
| Matrix metallopeptidase 8 | Mmp8 | 1.90 | 0.1009 | 1.26 | 0.9574 | 1.13 | 0.9999 |
| Matrix metallopeptidase 9 | Mmp9 | 1.91 | 0.1663 | 1.52 | 0.3911 | 1.24 | 0.9028 |
| Neural cell adhesion molecule 1 | Ncam1 | 0.56 | 0.0071† | 0.85 | 0.4708 | 0.33 | 0.0003† |
| Neural cell adhesion molecule 2 | Ncam2 | ND | - | ND | - | ND | - |
| Platelet/endothelial cell adhesion molecule 1 | Pecam1 | 1.01 | 0.9991 | 1.11 | 0.6413 | 1.20 | 0.1953 |
| Periostin, osteoblast specific factor | Postn | 0.62 | 0.0017† | 0.86 | 0.2503 | 0.38 | <0.0001† |
| Selectin, endothelial cell | Sele | 0.83 | 0.6299 | 1.22 | 0.5334 | 1.71 | 0.0076† |
| Selectin, lymphocyte | Sell | ND | - | ND | - | ND | - |
| Selectin, platelet | Selp | 0.99 | 0.9978 | 0.69 | 0.0193† | 1.02 | >0.9999 |
| Sarcoglycan, epsilon | Sgce | 1.57 | 0.0127† | 0.98 | 0.9993 | 1.27 | 0.357 |
| Secreted acidic cysteine rich glycoprotein | Sparc | 1.19 | 0.1279 | 0.92 | 0.6975 | 1.05 | 0.9429 |
| Sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1 | Spock1 | ND | - | ND | - | ND | - |
| Secreted phosphoprotein 1 | Spp1 | 1.59 | 0.6204 | 2.31 | 0.028† | 1.39 | 0.7043 |
| synaptotagmin 1 | Syt1 | 1.72 | 0.8436 | 1.12 | 0.9061 | 0.92 | 0.9725 |
| Transforming growth factor, beta induced | Tgfbi | 0.68 | 0.0226† | 1.27 | 0.0538† | 0.72 | 0.0414† |
| Thrombospondin 1 | Thbs1 | 0.90 | 0.4343 | 0.76 | 0.0224† | 0.70 | 0.0045† |
| Thrombospondin 2 | Thbs2 | 0.52 | 0.0658 | 0.89 | 0.8188 | 0.56 | 0.0745 |
| Thrombospondin 3 | Thbs3 | 1.66 | 0.0525† | 1.30 | 0.8955 | 0.97 | >0.9999 |
| Tissue inhibitor of metalloproteinase 1 | Timp1 | 1.10 | 0.8511 | 0.81 | 0.5181 | 0.99 | >0.9999 |
| Tissue inhibitor of metalloproteinase 2 | Timp2 | 1.12 | 0.7962 | 1.51 | 0.0167† | 1.59 | 0.0067† |
| Tissue inhibitor of metalloproteinase 3 | Timp3 | 0.77 | 0.3058 | 0.43 | 0.0078† | 0.30 | 0.0016† |
| Tenascin C | Tnc | 0.64 | 0.0067† | 0.80 | 0.1059 | 0.72 | 0.0196† |
| Vascular cell adhesion molecule 1 | Vcam1 | 1.87 | 0.0008† | 1.00 | 0.9944 | 1.62 | 0.0221† |
| Versican | Vcan | 0.61 | 0.0092† | 1.06 | 0.9172 | 0.84 | 0.3146 |
| Vitronectin | Vtn | ND | - | ND | - | ND | - |
†
P value <0.05.
ND-Not Detected