Table 2:
Baseline extracellular matrix gene expression levels in cardiac fibroblasts from fibroblast-specific Smad2 KO (FS2KO) mice.
| Gene name | Gene symbol | Smad2 fl/fl (mean±SEM) | FS2KO (mean±SEM) | p-value |
|---|---|---|---|---|
| A disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1motif, 1 | Adamts1 | 0.05011±0.004495 | 0.05468±0.002875 | 0.4241 |
| A disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1motif, 2 | Adamts2 | 0.1242±0.008133 | 0.1495±0.01546 | 0.1978 |
| A disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1motif, 5 | Adamts5 | 0.07718±0.01298 | 0.1003±0.01372 | 0.2665 |
| A disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1motif, 8 | Adamts8 | 4.250e-005±4.787e-006 | 6.750e-005±1.702e-005 | 0.2070 |
| CD44 antigen | Cd44 | 0.1194±0.003434 | 0.1327±0.007079 | 0.1422 |
| Cadherin 1 | Cdh1 | ND | ND | - |
| Cadherin 2 | Cdh2 | 0.01873±0.003922 | 0.02185±0.002256 | 0.5166 |
| Cadherin 3 | Cdh3 | 0.0001700±3.162e-005 | 0.0001100±2.799e-005 | 0.2052 |
| Cadherin 4 | Cdh4 | ND | ND | - |
| Contactin 1 | Cntn1 | ND | ND | - |
| collagen type I, alpha1 | Col1a1 | 1.322±0.09085 | 1.298±0.06676 | 0.8358 |
| collagen type II, alpha1 | Col2a1 | 0.01105±0.004770 | 0.004953±0.002213 | 0.2907 |
| collagen type III, alpha1 | Col3a1 | 3.325±0.2212 | 3.958±0.5178 | 0.3038 |
| collagen type IV, alpha1 | Col4a1 | 0.4297±0.03268 | 0.4059±0.04170 | 0.6693 |
| collagen type IV, alpha2 | Col4a2 | 0.1384±0.008707 | 0.1169±0.009966 | 0.1554 |
| collagen type IV, alpha3 | Col4a3 | 0.0001200±2.614e-005 | 0.0001700±4.163e-005 | 0.3484 |
| collagen type V, alpha1 | Col5a1 | 0.1914±0.01411 | 0.1818±0.009520 | 0.5945 |
| collagen type VI, alpha1 | Col6a1 | 0.3600±0.04680 | 0.3853±0.03039 | 0.6657 |
| Connective tissue growth factor | Ctgf | 2.578±0.2733 | 2.827±0.1393 | 0.4485 |
| Catenin (Cadherin associated protein), alpha1 | Ctnna1 | 0.08657±0.008150 | 0.1025±0.007268 | 0.1950 |
| Catenin (Cadherin associated protein), alpha2 | Ctnna2 | 0.05011±0.004495 | 0.05468±0.002875 | 0.4241 |
| Catenin (Cadherin associated protein), beta1 | Ctnnb1 | 0.1322±0.005469 | 0.1472±0.006247 | 0.1213 |
| Extracellular matrix protein 1 | Ecm1 | 0.05011±0.004495 | 0.05468±0.002875 | 0.4241 |
| Elastin microfibril interfacer 1 | Emilin1 | 0.08706±0.006891 | 0.09406±0.004824 | 0.4369 |
| Ectonucleoside triphosphate diphosphohydrolase 1 | Entpd1 | 0.05011±0.004495 | 0.05468±0.002875 | 0.4241 |
| Fibulin 1 | Fbln1 | 0.008356±0.001318 | 0.007695±0.001472 | 0.7491 |
| Fibronectin 1 | Fn1 | 0.05011±0.004495 | 0.05468±0.002875 | 0.4241 |
| Hyaluronan and proteoglycan link protein 1 | Hapln1 | ND | ND | - |
| Hemolytic complement | Hc | ND | ND | - |
| Intercellular adhesion molecule 1 | Icam1 | 0.04526±0.003715 | 0.08703±0.008140 | 0.0034† |
| Integrin alpha2 | Itga2 | 0.05011±0.004495 | 0.05468±0.002875 | 0.4241 |
| Integrin alpha3 | Itga3 | 0.006343±0.0004632 | 0.006505±0.0006678 | 0.8489 |
| Integrin alpha4 | Itga4 | 0.004250±0.0006292 | 0.008163±0.001345 | 0.0388† |
| Integrin alpha5 | Itga5 | 0.07631±0.01056 | 0.06432±0.003967 | 0.3287 |
| Integrin alpha1, epithelial-associated | Itgae | ND | ND | - |
| Integrin alpha L | Itgal | 0.0002500±8.165e-006 | 0.0002975±3.705e-005 | 0.2922 |
| Integrin alpha M | Igam | 2.250e-005±9.465e-006 | 4.250e-005±1.887e-005 | 0.3801 |
| Integrin alpha V | Itgav | 0.07016±0.01001 | 0.07201±0.007132 | 0.8854 |
| Integrin alpha X | Igtax | 0.05011±0.004495 | 0.05468±0.002875 | 0.4241 |
| Integrin beta1 | Itgb1 | 0.3718±0.03817 | 0.4283±0.02015 | 0.2390 |
| Integrin beta2 | Itgb2 | 0.05011±0.004495 | 0.05468±0.002875 | 0.4241 |
| Integrin beta3 | Itgb3 | 0.007356±0.0006389 | 0.007835±0.0005224 | 0.6857 |
| Integrin beta4 | Itgb4 | 0.05011±0.004495 | 0.05468±0.002875 | 0.4241 |
| Laminin,alpha1 | Lama1 | 0.0007726±7.470e-005 | 0.0006245±9.608e-005 | 0.6857 |
| Laminin,alpha2 | Lama2 | 0.008000±0.001080 | 0.008758±0.001548 | 0.7020 |
| Laminin,alpha3 | Lama3 | ND | ND | - |
| Laminin, beta2 | Lamb2 | 0.1242±0.008133 | 0.1495±0.01546 | 0.1978 |
| Laminin, beta3 | Lamb3 | 0.0001549±2.649e-005 | 0.0002531±9.019e-005 | 0.3365 |
| Laminin, gama1 | Lamc1 | 0.05011±0.004495 | 0.05468±0.002875 | 0.4241 |
| Matrix metallopeptidase 10 | Mmp10 | 0.0009981±0.0002581 | 0.0009728±5.866e-005 | 0.9291 |
| Matrix metallopeptidase 11 | Mmp11 | 0.05011±0.004495 | 0.05468±0.002875 | 0.4241 |
| Matrix metallopeptidase 12 | Mmp12 | 0.0001067±2.493e-005 | 0.0001167±4.918e-005 | 0.8617 |
| Matrix metallopeptidase 13 | Mmp13 | 0.05011±0.004495 | 0.05468±0.002875 | 0.4241 |
| Matrix metallopeptidase 14 | Mmp14 | 0.07840±0.007007 | 0.07942±0.004432 | 0.9059 |
| Matrix metallopeptidase 15 | Mmp15 | 0.0007500±0.0002500 | 0.001201±0.0001180 | 0.2857 |
| Matrix metallopeptidase I, alpha | Mmp1a | 0.0005438±0.0001033 | 0.0004808±7.519e-005 | 0.6394 |
| Matrix metallopeptidase 2 | Mmp2 | 0.04275±0.006897 | 0.04616±0.003666 | 0.6781 |
| Matrix metallopeptidase 3 | Mmp3 | 0.1488±0.03419 | 0.1464±0.03906 | 0.9641 |
| Matrix metallopeptidase 7 | Mmp7 | ND | ND | - |
| Matrix metallopeptidase 8 | Mmp8 | 0.001260±0.0001659 | 0.001073±0.0002067 | 0.5064 |
| Matrix metallopeptidase 9 | Mmp9 | ND | ND | - |
| Neural cell adhesion molecule 1 | Ncam1 | 0.01511±0.001621 | 0.01639±0.002848 | 0.7091 |
| Neural cell adhesion molecule 2 | Ncam2 | ND | ND | - |
| Platelet/endothelial cell adhesion molecule 1 | Pecam1 | 0.05011±0.004495 | 0.05468±0.002875 | 0.4241 |
| Periostin, osteoblast specific factor | Postn | 0.01174±0.002937 | 0.008773±0.001060 | 0.3781 |
| Selectin, endothelial cell | Sele | 0.05011±0.004495 | 0.05468±0.002875 | 0.4241 |
| Selectin, lymphocyte | Sell | ND | ND | - |
| Selectin, platelet | Selp | 0.02509±0.007001 | 0.04889±0.01727 | 0.3429 |
| Sarcoglycan, epsilon | Sgce | 0.03075±0.003301 | 0.03869±0.003668 | 0.1588 |
| Secreted acidic cysteine rich glycoprotein | Sparc | 3.999±0.08188 | 4.519±0.4423 | 0.3261 |
| Sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1 | Spock1 | ND | ND | - |
| Secreted phosphoprotein 1 | Spp1 | 0.1475±0.004519 | 0.1012±0.03215 | 0.2461 |
| synaptotagmin 1 | Syt1 | ND | ND | - |
| Transforming growth factor, beta induced | Tgfbi | 0.002250±0.0002500 | 0.002053±0.0004777 | 0.8000 |
| Thrombospondin 1 | Thbs1 | 1.907±0.1020 | 1.467±0.1256 | 0.0349† |
| Thrombospondin 2 | Thbs2 | 0.05250±0.008005 | 0.03626±0.002433 | 0.2000 |
| Thrombospondin 3 | Thbs3 | 0.002192±0.0001814 | 0.001634±0.0002342 | 0.1087 |
| Tissue inhibitor of metalloproteinase 1 | Timp1 | 0.05011±0.004495 | 0.05468±0.002875 | 0.4241 |
| Tissue inhibitor of metalloproteinase 2 | Timp2 | 1.399±0.05310 | 1.565±0.1505 | 0.3375 |
| Tissue inhibitor of metalloproteinase 3 | Timp3 | 1.265±0.1647 | 1.547±0.1258 | 0.2228 |
| Tenascin C | Tnc | 0.1975±0.02316 | 0.1565±0.006735 | 0.1399 |
| Vascular cell adhesion molecule 1 | Vcam1 | 0.1053±0.01070 | 0.1123±0.008587 | 0.6282 |
| Versican | Vcan | 0.01832±0.005616 | 0.01193±0.0009556 | 0.3399 |
| Vitronectin | Vtn | ND | ND | - |
†
P value <0.05.
ND-Not Detected