Table 3:
Baseline extracellular matrix gene expression levels in cardiac fibroblasts from fibroblast-specific Smad3 KO (FS3KO) mice
| Gene name | Gene symbol | Smad3 fl/fl (mean±SEM) | FS3KO (mean±SEM) | p-value |
|---|---|---|---|---|
| A disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1motif, 1 | Adamts1 | 0.08552±0.008394 | 0.08235±0.004471 | 0.7504 |
| A disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1motif, 2 | Adamts2 | 0.2270±0.04090 | 0.1796±0.01951 | 0.3359 |
| A disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1motif, 5 | Adamts5 | 0.1784±0.02840 | 0.1528±0.01668 | 0.4669 |
| A disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1motif, 8 | Adamts8 | 5.750e-005±1.109e-005 | 5.750e-005±1.315e-005 | >0.9999 |
| CD44 antigen | Cd44 | 0.1203±0.008070 | 0.1126±0.007737 | 0.5179 |
| Cadherin 1 | Cdh1 | ND | ND | - |
| Cadherin 2 | Cdh2 | 0.03067±0.004226 | 0.02008±0.001042 | 0.0510 |
| Cadherin 3 | Cdh3 | 0.0001100±2.273e-005 | 8.250e-005±3.065e-005 | 0.4982 |
| Cadherin 4 | Cdh4 | ND | ND | - |
| Contactin 1 | Cntn1 | ND | ND | - |
| collagen type I, alpha1 | Col1a1 | 1.584±0.2012 | 1.129±0.03113 | 0.0571 |
| collagen type II, alpha1 | Col2a1 | 0.01022±0.006811 | 0.0003875±0.0001616 | 0.0286† |
| collagen type III, alpha1 | Col3a1 | 6.392±0.8426 | 4.811±0.4546 | 0.1496 |
| collagen type IV, alpha1 | Col4a1 | 0.6953±0.05561 | 0.5286±0.02332 | 0.0327† |
| collagen type IV, alpha2 | Col4a2 | 0.1737±0.01462 | 0.1355±0.004078 | 0.0457† |
| collagen type IV, alpha3 | Col4a3 | 0.0001325±1.493e-005 | 7.000e-005±8.165e-006 | 0.0104† |
| collagen type V, alpha1 | Col5a1 | 0.2166±0.03011 | 0.1440±0.009342 | 0.0609 |
| collagen type VI, alpha1 | Col6a1 | 0.4923±0.03000 | 0.4311±0.03351 | 0.2223 |
| Connective tissue growth factor | Ctgf | 2.001±0.05805 | 1.694±0.2973 | 0.3795 |
| Catenin (Cadherin associated protein), alpha1 | Ctnna1 | 0.1082±0.008349 | 0.07824±0.004927 | 0.0215† |
| Catenin (Cadherin associated protein), alpha2 | Ctnna2 | 0.08552±0.008394 | 0.08235±0.004471 | 0.7504 |
| Catenin (Cadherin associated protein), beta1 | Ctnnb1 | 0.1350±0.006237 | 0.1192±0.005547 | 0.1065 |
| Extracellular matrix protein 1 | Ecm1 | 0.08552±0.008394 | 0.08235±0.004471 | 0.7504 |
| Elastin microfibril interfacer 1 | Emilin1 | 0.1401±0.01728 | 0.1160±0.005231 | 0.2288 |
| Ectonucleoside triphosphate diphosphohydrolase 1 | Entpd1 | 0.08552±0.008394 | 0.08235±0.004471 | 0.7504 |
| Fibulin 1 | Fbln1 | 0.006493±0.001556 | 0.004165±0.0005900 | 0.2114 |
| Fibronectin 1 | Fn1 | 0.08552±0.008394 | 0.08235±0.004471 | 0.7504 |
| Hyaluronan and proteoglycan link protein 1 | Hapln1 | ND | ND | - |
| Hemolytic complement | Hc | ND | ND | - |
| Intercellular adhesion molecule 1 | Icam1 | 0.05568±0.004796 | 0.05175±0.002562 | 0.4975 |
| Integrin alpha2 | Itga2 | 0.08552±0.008394 | 0.08235±0.004471 | 0.7504 |
| Integrin alpha3 | Itga3 | 0.003108±0.0003279 | 0.002097±0.0005673 | 0.1739 |
| Integrin alpha4 | Itga4 | 0.008000±0.001954 | 0.006000±0.0009129 | 0.3895 |
| Integrin alpha5 | Itga5 | 0.04811±0.007806 | 0.03566±0.001261 | 0.2090 |
| Integrin alpha1, epithelial-associated | Itgae | ND | ND | - |
| Integrin alpha L | Itgal | 0.0005550±0.0001202 | 0.002135±0.0004533 | 0.0151† |
| Integrin alpha M | Itgam | 0.007605±0.0006380 | 0.02065±0.006379 | 0.1330 |
| Integrin alpha V | Itgav | 0.07837±0.008046 | 0.06176±0.002823 | 0.0994 |
| Integrin alpha X | Igtax | 0.08552±0.008394 | 0.08235±0.004471 | 0.7504 |
| Integrin beta1 | Itgb1 | 0.4998±0.04867 | 0.3983±0.03534 | 0.1425 |
| Integrin beta2 | Itgb2 | 0.08552±0.008394 | 0.08235±0.004471 | 0.7504 |
| Integrin beta3 | Itgb3 | 0.006515±0.0007208 | 0.005433±0.0005200 | 0.2691 |
| Integrin beta4 | Itgb4 | 0.08552±0.008394 | 0.08235±0.004471 | 0.7504 |
| Laminin,alpha1 | Lama1 | 0.0006850±0.0004440 | 0.0003373±0.0001388 | 0.8857 |
| Laminin,alpha2 | Lama2 | 0.01510±0.002387 | 0.01050±0.001041 | 0.1276 |
| Laminin,alpha3 | Lama3 | ND | ND | - |
| Laminin, beta2 | Lamb2 | 0.2270±0.04090 | 0.1796±0.01951 | 0.3359 |
| Laminin, beta3 | Lamb3 | 0.0003100±5.642e-005 | 0.0003403±4.560e-005 | 0.6912 |
| Laminin, gama1 | Lamc1 | 0.08552±0.008394 | 0.08235±0.004471 | 0.7504 |
| Matrix metallopeptidase 10 | Mmp10 | 0.0009200±0.0002996 | 0.0007273±7.612e-005 | 0.5724 |
| Matrix metallopeptidase 11 | Mmp11 | 0.08552±0.008394 | 0.08235±0.004471 | 0.7504 |
| Matrix metallopeptidase 12 | Mmp12 | 0.02601±0.0005370 | 0.02907±0.007396 | 0.7067 |
| Matrix metallopeptidase 13 | Mmp13 | 0.08552±0.008394 | 0.08235±0.004471 | 0.7504 |
| Matrix metallopeptidase 14 | Mmp14 | 0.08783±0.006574 | 0.08574±0.006208 | 0.8857 |
| Matrix metallopeptidase 15 | Mmp15 | 0.0005925±7.157e-005 | 0.0005000±0.0002887 | >0.9999 |
| Matrix metallopeptidase I, alpha | Mmp1a | 0.007983±0.002195 | 0.006278±0.001565 | 0.5505 |
| Matrix metallopeptidase 2 | Mmp2 | 0.04454±0.006701 | 0.04175±0.001250 | 0.7081 |
| Matrix metallopeptidase 3 | Mmp3 | 0.3447±0.08269 | 0.3010±0.07823 | 0.7144 |
| Matrix metallopeptidase 7 | Mmp7 | ND | ND | - |
| Matrix metallopeptidase 8 | Mmp8 | 0.004628±0.001785 | 0.002308±0.0005025 | 0.2574 |
| Matrix metallopeptidase 9 | Mmp9 | ND | ND | - |
| Neural cell adhesion molecule 1 | Ncam1 | 0.01641±0.003805 | 0.008610±0.0006935 | 0.1313 |
| Neural cell adhesion molecule 2 | Ncam2 | ND | ND | - |
| Platelet/endothelial cell adhesion molecule 1 | Pecam1 | 0.08552±0.008394 | 0.08235±0.004471 | 0.7504 |
| Periostin, osteoblast specific factor | Postn | 0.01017±0.001744 | 0.005487±0.001245 | 0.0714 |
| Selectin, endothelial cell | Sele | 0.08552±0.008394 | 0.08235±0.004471 | 0.7504 |
| Selectin, lymphocyte | Sell | ND | ND | - |
| Selectin, platelet | Selp | 0.01438±0.001676 | 0.008881±0.0005781 | 0.0211† |
| Sarcoglycan, epsilon | Sgce | 0.03166±0.002014 | 0.03925±0.002428 | 0.0571 |
| Secreted acidic cysteine rich glycoprotein | Sparc | 4.222±0.3581 | 3.930±0.2464 | 0.5262 |
| Sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1 | Spock1 | ND | ND | - |
| Secreted phosphoprotein 1 | Spp1 | 0.2215±0.04578 | 0.08450±0.03218 | 0.0499† |
| synaptotagmin 1 | Syt1 | ND | ND | - |
| Transforming growth factor, beta induced | Tgfbi | 0.002168±0.0003201 | 0.004750±0.001031 | 0.0538 |
| Thrombospondin 1 | Thbs1 | 2.199±0.2340 | 1.497±0.1276 | 0.0389† |
| Thrombospondin 2 | Thbs2 | 0.05461±0.01732 | 0.03425±0.005618 | 0.6857 |
| Thrombospondin 3 | Thbs3 | 0.0009700±0.0001891 | 0.0006808±0.0001137 | 0.2378 |
| Tissue inhibitor of metalloproteinase 1 | Timp1 | 0.08552±0.008394 | 0.08235±0.004471 | 0.7504 |
| Tissue inhibitor of metalloproteinase 2 | Timp2 | 1.001±0.04551 | 1.126±0.1519 | 0.4593 |
| Tissue inhibitor of metalloproteinase 3 | Timp3 | 1.766±0.1795 | 1.133±0.07485 | 0.0173† |
| Tenascin C | Tnc | 0.1372±0.01384 | 0.1166±0.006884 | 0.2324 |
| Vascular cell adhesion molecule 1 | Vcam1 | 0.05594±0.009035 | 0.04700±0.002160 | 0.4002 |
| Versican | Vcan | 0.008155±0.001039 | 0.005431±0.0008986 | 0.0946 |
| Vitronectin | Vtn | ND | ND | - |
†
P value <0.05.
ND-Not Detected