Expression of protein involved in calcium handling in LMNA cardiomyopathy.
(A) Representative immunoblots showing total PLB, phosphorylated PLB(Ser16) and phosphorylated PLB(Thr17) expressions in hearts from 6 months-old male LmnaH222P/H222P and wild type (WT) mice. GAPDH was shown as loading control.
(B) Schematic representation of the construct.
(C) Representative immunoblots showing total PLB and phosphorylated PLB(Ser16) expressions in HL-1 cells stably expressing FLAG epitope-tagged SLN (FLAG-SLN).
(D) Micrographs showing PLB labeling in HL-1 cells (empty) and HL-1 cells stably expressing FLAG epitope-tagged SLN (FLAG-SLN). Nuclei are counter-stained with 4′,6-diamidino-2-phenylindole (dapi).