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. 2020 May 18;22:100767. doi: 10.1016/j.bbrep.2020.100767

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Expression of protein involved in calcium handling in LMNA cardiomyopathy.

(A) Representative immunoblots showing total PLB, phosphorylated PLB(Ser₁₆) and phosphorylated PLB(Thr₁₇) expressions in hearts from 6 months-old male Lmna^H222P/H222P and wild type (WT) mice. GAPDH was shown as loading control.

(B) Schematic representation of the construct.

(C) Representative immunoblots showing total PLB and phosphorylated PLB(Ser₁₆) expressions in HL-1 cells stably expressing FLAG epitope-tagged SLN (FLAG-SLN).

(D) Micrographs showing PLB labeling in HL-1 cells (empty) and HL-1 cells stably expressing FLAG epitope-tagged SLN (FLAG-SLN). Nuclei are counter-stained with 4′,6-diamidino-2-phenylindole (dapi).