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. 2020 May 31;7:39. doi: 10.1186/s40634-020-00256-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — TGFβ staining on the medial tibial surface. a Uninjected left knee. Arrowhead identifies bone marrow. Note normal immunopositivity in connective tissue of synovium (S), collateral ligament joint capsule, and cruciates (small C). b PBS injected right knee. Note increased connective tissue staining in healing medial synovium (S) damaged meniscus (M), cruciates (small C), and osteophyte (OP). The damaged tibial cartilage has increased staining of the tangential layer in areas where it is intact. c IA-BioHA (0.5 mg/rat) injected at Days 7, 14, and 21 in the right knee. Note increased connective tissue staining in healing medial synovium (S) damaged meniscus (M), cruciates (small C), and osteophyte (OP). The damaged tibial cartilage has increased staining of the tangential layer in areas where it is intact. F- femur; M – meniscus; T – tibia. Images were acquired at 25x magnification