. 2020 Jun 1;21:14. doi: 10.1186/s40510-020-00314-8

Table 1.

Growth conditions of each bacterial species for the multi-species biofilm model

Bacterial species	Growth condition
Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguinis, Streptococcus salivarius, Streptococcus oralis, Actinomyces naeslundii, Lactobacillus rhamnosus, Veillonella dispar, Neisseria subflava	Brain heart infusion medium at 37 °C with 5% CO₂
Fusobacterium nucleatum, Prevotella nigrescens, Porphyromonas gingivalis	Anaerobic condition with tryptic soy agar medium supplemented with 10 μg/mL vitamin K, 5 μg/mL hemin, and 5% sheep blood at 37 °C for 7 days Subcultured in BHI medium with 10 μg/mL vitamin K and 5 μm/mL hemin and grown to mid-exponential phase anaerobically at 37 °C
Actinobacillus actinomycetemcomitans	Brain heart infusion medium at 37 °C in an anaerobic condition

Bacterial species

Growth condition

Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguinis, Streptococcus salivarius, Streptococcus oralis, Actinomyces naeslundii, Lactobacillus rhamnosus, Veillonella dispar, Neisseria subflava

Brain heart infusion medium at 37 °C with 5% CO₂

Fusobacterium nucleatum, Prevotella nigrescens, Porphyromonas gingivalis

Anaerobic condition with tryptic soy agar medium supplemented with 10 μg/mL vitamin K, 5 μg/mL hemin, and 5% sheep blood at 37 °C for 7 days

Subcultured in BHI medium with 10 μg/mL vitamin K and 5 μm/mL hemin and grown to mid-exponential phase anaerobically at 37 °C

Actinobacillus actinomycetemcomitans

Brain heart infusion medium at 37 °C in an anaerobic condition