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. 2020 Jun 1;7:88. doi: 10.1038/s41438-020-0311-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a–c Phenotype (a), chlorophyll content (b) and MDA content (c) of three-month-old diploid and tetraploid plants after two weeks of culture in salt solution or water (control). d Phenotype of seven-month-old potted seedlings after 30 days of irrigation with salt solution or water (control). e–g Phenotype (e), chlorophyll content (f) and electrolyte leakage (g) of fresh shoots obtained from 2-year-old diploid and tetraploid plants before and/or after salt treatment. h Trypan Blue staining of diploid and tetraploid leaves with or without (control) salt treatment. Error bars indicate SE (n = 3). Asterisks indicate significant differences between the diploids and tetraploids (*P < 0.05; **P < 0.01; *** P< 0.001)